规格
Final Product Type:	Plasmid DNA
For Use With (Application):	PCR, Cloning, Southern Blotting, Sequencing, Transformation
Purification Target:	Plasmid DNA
Sample Type:	Yeast
Isolation Technology:	ChargeSwitch® Chemistry
High-throughput Compatibility:	Not High-throughput Compatible (Manual)
Prep Scale:	< 100 µg (Small-Scale) Plasmid DNA
Shipping Condition:	Room Temperature
Product Line:	ChargeSwitch®

储存

All components of the ChargeSwitch® Plasmid Yeast Mini Kit are shipped at room temperature. Upon receipt, store components as follows: Mix RNase A in Resuspension Buffer (R4) and store RNase A in Resuspension Buffer (R4) at 4“C. Store Precipitation Buffer (N5) at 4“C to improve performance. Store the remaining kit components at room temperature. All components are guaranteed stable for 6 months when stored properly.

Components:

ChargeSwitch® Magnetic Beads (25 mg/ml in 10 mM MES, pH 5.0, 10 mM NaCl, 0.1% Tween 20)
RNase A (5 mg/ml in 10 mM Tris-HCl, pH 8.5, 10 mM EDTA)
ChargeSwitch® Resuspension Buffer (R4)
ChargeSwitch® Lysis Buffer (L9)
ChargeSwitch® Precipitation Buffer (N5)
ChargeSwitch® Wash Buffer (W11)
ChargeSwitch® Wash Buffer (W12)
ChargeSwitch® Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)

描述

Sample size 1 ml S. cerevisiae culture; Typical yield up to 25 ng

• Increased transformation efficiency
• Reagents that avoid PCR inhibition
• Purifies DNA with improved downstream performance

The ChargeSwitch® Plasmid Yeast Mini Kit provides an efficient method for purifying high-quality plasmid DNA from 1 ml of Saccharomyces cerevisiae grown in selective minimal media. This kit typically yields up to 25 ng of plasmid DNA. Data generated from transformation and PCR experiments using ChargeSwitch® purified DNA shows improved performance versus DNA purified by other methods (Figure 1). This kit includes all the necessary reagents for the number of preps listed and requires the use of a magnetic accessory. Please see the Accessories Chapter (Chapter 6) to select the best magnetic accessory for your needs.

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