规格
Accession Number:	NM_000524
Assay Entry:	Cell-based beta-arrestin recruitment
Cell Line:	U2OS
Cell State:	Dividing Cells
Cytoplasmic Tail:	Tail from AVPR2 GPCR
Druggable Target:	GPCRs
Gene Symbol:	HTR1A
Human Protein Class:	G-Protein Coupled Receptor (GPCR)
Population Status:	Single Cell Clone Origin
Product Line:	Tango™
Readout:	End Point
System Type:	Tango™
Target Entry:	5HT1A, HTR1A
Technique:	FRET
Detection Method:	Fluorescent

储存

K1857 Tango™ HTR1A-UAS-bla CHO-K1 cells
(LiveBLAzer™-FRET B⁄G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:
• Tango™ HTR1A-UAS-bla CHO-K1 cells
• Tango™ HTR1A-UAS-bla CHO-K1 Assay Protocol
• Certificate of Analysis
The Tango™ HTR1A-UAS-bla CHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.

描述

Tango™ HTR1A-bla U2OS cells contain the human Serotonin Type 1a receptor (HTR1A) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin⁄TEV protease fusion protein and the beta-lactamase reporter gene under the control of a UAS response element. Tango™ HTR1A-bla U2OS DA cells have been functionally validated for Z’ factor and EC50 concentrations of 5-CT (Figure 1). In addition, Tango™ HTR1A-bla U2OS cells have been tested for assay performance under variable conditions.

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