规格
|
Assay Entry:
|
Biochemical binding/kinetic |
Kinase Group:
|
Serine/Threonine Kinase |
Species:
|
Human |
Target Entry:
|
PRKACG (PRKAC gamma) |
Molecular Weight:
|
66.7 kDa |
Gene Alias:
|
KAPG, PKAr, cAPKr |
Validated Application:
|
Kinase Assay |
Protein Tag:
|
GST Tag |
储存
|
Storage Buffer: 50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.1 mM EGTA, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol
Store at -68 to -85°C. Aliquot at first use to avoid multiple freeze-thaws. |
PRKACG (PRKAC gamma) is a third isoform of the catalytic subunit of cAMP-dependent protein kinase. It was isolated from a human testis cDNA library and was clearly derived from a gene distinct from C-alpha and C-beta and showed tissue-specific expression. Whereas at the amino acid level C-alpha and C-beta showed 93% homology, C-gamma showed only about 80% homology to both C-alpha and C-beta. The PRKACG gene is intronless, contains remnants of a poly(A) tail, is flanked by direct repeats, and is co-linear with the PRKACA gene. Recombinant full-length human PRKACG was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag.
• Validated in LanthaScreen® Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use
Related Product:
LanthaScreen® Eu Kinase Binding Assay