规格
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For Use With (Equipment):
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Floid® Cell Imaging System, Fluorescence Microscope, Flow Cytometer, High Content Instrument |
Form:
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Frozen |
Indicator:
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Lipid Peroxidation Sensor |
Product Line:
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Image-iT® |
Shipping Condition:
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Wet Ice |
Solubility:
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DMSO (Dimethylsulfoxide) |
Sub Cellular Localization:
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Cytoplasm |
Color:
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Green |
Detection Method:
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Fluorescent |
储存
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Store component A store at ≤-20°C
Store component B at room temperature |
The Image-iT® Lipid Peroxidation Kit enables the detection of lipid peroxidation in live cells through oxidation of BODIPY® 581/591 C11 reagent. This reagent localizes to membranes throughout live cells and upon oxidation by lipid hydroperoxides, displays a shift in peak fluorescence emission from ~590 nm to ~510 nm. Fluorescence from live cells shifts from red to green, providing a ratiometric indication of lipid peroxidation compatible with traditional and high content microscopy, as well as flow cytometry. This kit also includes cumene hydroperoxide as a positive control compound to induce lipid peroxidation in cells.
Additional Information:
• The reagent is provided as a stable, ready-to-use DMSO solution with a simple protocol compatible with standard workflows in fluorescence microscopy
• The reagent is provided as five single-use vials containing sufficient total reagent for five 96-well plates or 100 coverslips
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.