MACSPlex EV Kit Neuro, human，130-133-428，美天旎

The MACSPlex EV Kit Neuro (for neurobiology) allows the detection and characterization of extracellular vesicles (EVs), such as exosomes, using 37 different surface epitopes and two isotype controls. The MACSPlex EV Kit Neuro comprises a cocktail of various fluorescently labeled bead populations, each coated with a specific antibody binding the respective surface epitopes. The 39 bead populations can be distinguished by different fluorescence intensities via flow cytometry.

Extracellular vesicles (EVs) are incubated with the antibody-coated MACSPlex EV Capture Beads. Subsequently or in parallel, EVs bound to the MACSPlex EV Capture Beads are labeled with the MACSPlex EV Detection Reagents. The MACSPlex EV Detection Reagents can also be combined to create a cocktail comprising of MACSPlex EV Detection Reagent for CD9, CD63, and CD81. Consequently, sandwich complexes are formed between the MACSPlex EV Capture Bead, exosome, and the detection reagent (see Data and Images, Figure 1). These complexes can be analyzed based on the fluorescence characteristics of both the MACSPlex EV Capture Bead and the detection reagent. Positive signals indicate the presence of the respective surface epitope within the EV population (see Data and Images, Figure 2). It is also possible to compare different EV samples using the MACSPlex EV Kits allowing semi-quantitative analysis of differential surface epitopes.