Naive CD4+ T Cell Iso Kit II, human，130-094-131，美天旎

The Naive CD4⁺ T Cell Isolation Kit II was developed for the isolation of untouched naive CD4⁺ T cells from human peripheral blood mononuclear cells.

For magnetic depletion of memory CD4⁺ T cells and non-CD4⁺ T cells, PBMCs are incubated with a cocktail of biotinylated CD45RO, CD8, CD14, CD15, CD16, CD19, CD25, CD34, CD36, CD56, CD123, anti-TCRγ/δ, anti-HLA-DR, and CD235a (glycophorin A) antibodies. These cells are subsequently magnetically labeled with Anti-Biotin MicroBeads for depletion.

Naive CD4⁺ T cells isolated with the Naive CD4⁺ T Cell Isolation Kit II can be used when direct labeling of cell surface molecules could interfere with downstream applications, e.g., in studies on naive T helper cell activation and in functional studies of DCs.^{1, 2} Untouched naive CD4⁺ T cells can also be used for further isolation of specific T helper cell subsets.

For isolation of naive CD4⁺ recent thymic emigrants the CD4⁺ Recent Thymic Emigrant Isolation Kit, human is recommended.

LS, XS, or autoMACS^® Columns.