规格
Agent:	Blasticidin
Formula Weight:	458.9 g⁄mole
Product Line:	Gibco®
Reagent Type:	Antibiotic (Selective)
Shipping Condition:	Dry Ice
Validated Application:	Eukaryotic Selection⁄Stable Cell Line Generation, Bacterial Selection
Form:	Liquid
Concentration:	10 mg⁄ml, 10 mg⁄mL, 10 mg⁄ml
Quantity:	1 bottle
Sterility:	Sterile

储存
Storage conditions: -5°C to -20°C (protect from light) Shipping conditions: Frozen Shelf life: 9 months from date of manufacture

描述

Blasticidin S is a nucleoside antibiotic isolated from Streptomyces griseochromogenes. It is a potent inhibitor of protein synthesis in both prokaryotic and eukaryotic cells. Blasticidin S acts quickly by inhibiting peptide bond formation by the ribosome. Blasticidin S is used for the prevention of contamination of cell cultures. The recommended working concentration ranges from 2 to 20 µg/ml depending on the cell line. This product is provided as a solution at 10 mg/ml in 20 mM HEPES (pH 7.2-7.5). Life Technologies offers a variety of antibiotics and antimycotics for cell culture applications.

In addition, Blasticidin S is used as a bacterial selective antibiotic in the concentration range of 50–100 µg/ml. Resistance is conferred by expression of either one of two Blasticidin S deaminase genes: BSD (2) or bsr.These deaminases convert Blasticidin S to a nontoxic derivative. For selection purposes, the salt content of the LB medium must remain low (<90 mM) and the pH should not exceed 7.0 to maintain the activity of Basticidin S. Life Technologies also offers a variety of selection antibiotics.

Product Use

For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

cGMP Manufacturing
Gibco® Blasticidin S HCl is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

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