Molecular Probes® fibrinogen conjugates are prepared by attaching fluorescent dye molecules to purified human fibrinogen (approximately 15 dye molecules for each fibrinogen molecule), purifying the conjugate to remove unreacted dye, and then lyophilizing for storage.

Fluorescently labeled fibrinogen has proven to be a valuable tool for investigating platelet activation and subsequent fibrinogen binding. For instance, fluorescein-labeled fibrinogen has been used to detect fibrinogen bound to activated platelets by flow cytometry.

Human Fibrinogen Conjugate Specifications:
• Label (Ex/Em): Oregon Green® 488 (~496/524)
• Spectrally similar to fluorescein, but the signal is less pH-dependent and more photostable
• Lyophilized product can be dissolved in buffer (e.g., sodium bicarbonate, pH 8.3) for use
• Fluorescence is typically detected using fluorescence microscopy or flow cytometry

Find More Probes for Cell Adhesion and Receptor Binding
Review Probes for Cell Adhesion, Chemotaxis, Multidrug Resistance and Glutathione—Section 15.6 and Probes for Following Receptor Binding and Phagocytosis—Section 16.1 in the Molecular Probes® Handbook for more information on these probes.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

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