规格
Alternate Agonists:	Docosahexaenoic Acid, Phytanic Acid, All-trans-retinoic acid
Antagonists:	none
Assay Entry:	Cell-based beta lactamase reporter gene
Cell Line:	293T (HEK)
Cell State:	Division-Arrested Cells
Druggable Target:	Nuclear Receptors
Gene Symbol:	RARβ
Primary Agonist:	9-cis-retinoic acid
Reporter Gene:	BLA (Beta-Lactamase)
System Type:	GeneBLAzer™
Target Entry:	RAR beta, NR1B2, RARB
Detection Method:	Fluorescent

储存
Shipping: Dry IceStorage: -80°C/Liquid NitrogenSufficient division-arrested cells and substrate to assay one 384-well plate. Includes:RAR beta HEK 293T DA cells (K1407A)LiveBLAzer™-FRET B/G Loading Kit, 70 μg

描述

GeneBLAzer®RAR beta DA (Division Arrested) cells and RAR beta-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human retinoic acid receptor beta (RAR beta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer®UAS-bla HEK 293T cell line. GeneBLAzer®UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-RAR beta (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both RAR beta DA cells and RAR beta-UAS-bla HEK 293T cells are functionally validated for Z' and EC₅₀ concentrations of all-trans retinoic acid (ATRA). In addition, RAR beta-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, and stimulation time.

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