规格
|
Label Type:
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Other Label(s) or Dye(s) |
Product Line:
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CellTracker™ |
Reagent Type:
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Cell Tracker Compounds, Cell Labeling Reagents |
Shipping Condition:
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Room Temperature |
Quantity:
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1mg |
储存
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Store in freezer (-5 to -30°C). |
CellTracker™ Green CMFDA (5-chloromethylfluorescein diacetate) is a fluorescent dye well suited for monitoring cell movement or location. After loading into cells, the dye is well retained, allowing for multigenerational tracking of cellular movements. The green excitation/emission spectra are ideal for multiplexing with red fluorescent dyes and proteins.
Need a different emission spectrum or longer tracking? View our other mammalian cell tracking products.
• Easy to use—remove medium, add dye, incubate 30 minutes, and image cells
• Fluorescent signal retention of >72 hours (typically three to six generations)
• Green excitation/emission spectra (492/517 nm maxima) ideal for multiplexing
• Low cytotoxicity—does not affect viability or proliferation
CellTracker™ Green CMFDA fluorescent dye has been designed to freely pass through cell membranes into cells, where it is transformed into a cell-impermeant, fluorescent product. CellTracker™ Green fluorescent dyes are retained in living cells through several generations. The dyes are transferred to daughter cells but not adjacent cells in a population. CellTracker™ Green CMFDA dye is designed to display fluorescence for at least 72 hours, and the dye exhibits ideal tracking properties: it is stable, nontoxic at working concentrations, well retained in cells, and brightly fluorescent at physiological pH. Additionally, the excitation and emission spectra of CellTracker™ Green CMFDA dye are well separated from RFP (red fluorescent protein) spectra allowing for multiplexing.