Anti-TOM22 MicroBeads, mouse，130-127-693，美天旎

Anti-TOM22 Microbeads, mouse have been developed to be used in combination with the Mitochondria Isolation Kit, mouse tissue for the efficient isolation of functional and viable mitochondria. The isolation protocol is based on the renowned MACS Technology, which enables fast isolation of high purity and high yield mitochondria.

Tissues can be homogenized with the gentleMACS or gentleMACS Octo Dissociator in combination with the Mitochondria Extraction Kit – Tissue. After one-step homogenization of the tissue and lysis of the cell suspension, mitochondria are magnetically labeled with Anti-TOM22 MicroBeads, mouse, which bind to the translocase of the outer mitochondrial membrane 22 protein (TOM22). The sample is loaded onto an LS Column placed in a MidiMACS or QuadroMACS Separator. After washing, only the magnetically labeled mitochondria are retained on the column. The column is removed from the separator and functional mouse mitochondria are eluted from the column.

After isolation mitochondria can be further subjected to Western blot analysis^1,6 or downstream functional assays, including measurement of O2, membrane potential, and ATP^4-5,7, or respiratory control⁴. Growing evidence also suggests that isolated mitochondria are well suited for mitochondrial RNA expression profiling studies^2,3.