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INV110 OS KT 20 RXN,C717103,Invitrogen

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订货号 7UN3314
品牌型号 Invitrogen C717103
货期 询货期
最小订货量 1套
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产品介绍 Product Description
规格
Bacterial or Yeast Strain: INV110
Blue/White Screening: Yes
Cloning Methylated DNA: No
Cloning Unstable DNA: Not Suitable for cloning unstable DNA
Competent Cell Type: Chemically Competent
F'episome (to make ssDNA): Contains F' Episome
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Preparing Unmethylated DNA: Suitable for preparing unmethylated DNA
Product Line: One Shot™
Propagating ccdB Vectors: Not for ccdB vector propagation
Reduces Recombination: No
T1 Phage - Resistant (tonA): Yes
Transformation Efficiency: > 1x10^6
Transformation Efficiency Level: Subcloning Efficiency (10^6-10^7 cfu⁄µg)
Format: One Shot
Improves Plasmid Quality: Yes
Species: E. coli
储存
One Shot® INV110 Chemically Competent E. coli contains 10 transformations of chemically competent cells in One Shot® format. Store at -80°C.
描述

INV110 E. coli are derived from JM110 E. coli and are specifically designed for the growth and purification of plasmid DNA that will be digested with dam or dcm methylation-sensitive restriction enzymes. INV110 is a significant improvement over the JM110 strain. INV110 has been engineered to carry an endA1 mutation, which eliminates the non-specific endonuclease Endonuclease I, for improved plasmid DNA preparation. In addition, the INV110 genotype features:

• Blue/white screening of recombinant clones (lacZΔM15)
• Reduced cleavage of foreign methylated DNA for improved transformation of genomic DNA [Δ(mcrC-mrr)]
• F´ episome for phage infection and rescue of single-strand DNA

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