- 商品介绍
- 规格参数
- 包装参数
规格 |
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Bacterial or Yeast Strain: | INV110 |
Blue/White Screening: | Yes |
Cloning Methylated DNA: | No |
Cloning Unstable DNA: | Not Suitable for cloning unstable DNA |
Competent Cell Type: | Chemically Competent |
F'episome (to make ssDNA): | Contains F' Episome |
High-throughput Compatibility: | Not High-throughput Compatible (Manual) |
Preparing Unmethylated DNA: | Suitable for preparing unmethylated DNA |
Product Line: | One Shot™ |
Propagating ccdB Vectors: | Not for ccdB vector propagation |
Reduces Recombination: | No |
T1 Phage - Resistant (tonA): | Yes |
Transformation Efficiency: | > 1x10^6 |
Transformation Efficiency Level: | Subcloning Efficiency (10^6-10^7 cfu⁄µg) |
Format: | One Shot |
Improves Plasmid Quality: | Yes |
Species: | E. coli |
储存 |
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One Shot® INV110 Chemically Competent E. coli contains 10 transformations of chemically competent cells in One Shot® format. Store at -80°C. |
描述 |
INV110 E. coli are derived from JM110 E. coli and are specifically designed for the growth and purification of plasmid DNA that will be digested with dam or dcm methylation-sensitive restriction enzymes. INV110 is a significant improvement over the JM110 strain. INV110 has been engineered to carry an endA1 mutation, which eliminates the non-specific endonuclease Endonuclease I, for improved plasmid DNA preparation. In addition, the INV110 genotype features:
• Blue/white screening of recombinant clones (lacZΔM15)
• Reduced cleavage of foreign methylated DNA for improved transformation of genomic DNA [Δ(mcrC-mrr)]
• F´ episome for phage infection and rescue of single-strand DNA
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