- 商品介绍
- 规格参数
- 包装参数
产品介绍
Product Description
|
规格 |
|
| Cloning Method: | TOPO®-TA |
| Key Functions: | Reporter Assays, Clone your own Promoter |
| Product Line: | TOPO® |
| Delivery Type: | Transfection |
| Promoter: | None (Promoterless) |
| Reporter Gene: | GFP (Cycle 3) |
| Selection Agent (Eukaryotic): | Geneticin® (G-418) |
| Vector: | pGlow, TOPO-TA Vectors |
| Protein Tag: | Untagged |
|
储存 |
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| pGlow TOPO® TA Expression Kits contains two boxes. The TOPO® TA Cloning box contains linearized and topoisomerase I-activated pGlow-TOPO® vector, dNTPs, primers for sequencing, salt solution, and control PCR template and primers; store at -20°C. The One Shot® box contains One Shot® TOP10 E. coli cells, S.O.C. medium, and a supercoiled control plasmid; store at -80°C. The kits are guaranteed stable for 6 months when properly stored. |
|
描述 |
The pGlow TOPO® TA Expression Kit uses the Cycle 3 GFP mutant (1) as a reporter protein for transcriptional activity. Cycle 3 GFP is ideal for non-invasive analysis of promoter elements within intact, living cells. The fluorescent property of Cycle 3 GFP allows in vivo detection in virtually any cell type or species using microscopy with wild-type GFP filter sets or by fluorescence-activated cell sorting methods.
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