规格
|
Key Functions:
|
Regulated Expression |
Product Line:
|
T-REx™, pcDNA™ |
Shipping Condition:
|
Room Temperature |
Constitutive or Inducible System:
|
Inducible |
Delivery Type:
|
Transfection |
Promoter:
|
CMV |
Selection Agent (Eukaryotic):
|
Blasticidin |
Vector:
|
pcDNA |
Protein Tag:
|
Untagged |
储存
|
20 µg of supercoiled vector (40 µL at 0.5 µg/µL) supplied in TE Buffer, pH 8.0. Store at -20°C. |
The pcDNA™6⁄TR vector is part of the T-REx™ System (catalog K102001), which yields higher levels of induced expression than any other regulated mammalian expression system. It utilizes the complete CMV promoter and adds control elements from the bacterial tetracycline resistance operon to effectively repress and derepress transcription from one of the strongest mammalian promoter sequences known (1,2).
The regulatory vector, pcDNA™6⁄TR, is provided for high-level expression of the tetracycline repressor (TR) protein. This vector expresses the Blasticidin resistance gene for rapid selection of mammalian cell lines that stably express the TR protein.