规格
Final Product Type:	In Vitro Transcribed RNA (Purified)
For Use With (Application):	Microarray Analysis, Northern Blotting, Nuclease Protection Assays
For Use With (Equipment):	Vacuum Manifold, Centrifuge
Purification Target:	In Vitro Transcribed RNA
Sample Type:	In Vitro Transcribed RNA, Total RNA
Format:	96-well plate
Isolation Technology:	Silica Spin Column
No. of Reactions:	96 Preps
Yield:	100 µg
High-throughput Compatibility:	High-throughput Compatible
Purification Time:	30 min
Shipping Condition:	Room Temperature
Starting Material Amount:	1 ng to 500 µg of dsRNA>200b or ssRA>100bp
Product Line:	Ambion™, MEGAclear™

储存

• 8 ml Binding Solution (store at 4°C)
• 20 ml Wash Solution Concentrate (store at 4°C)
• 10 ml Elution Solution (store at room temperature, 4°C, or -20°C)
• 20 ml Nuclease-free Water (store at room temperature, 4°C, or -20°C)
• 1 MEGAclear™-96 Filter Plate (store at room temperature)
• 1 Collection Plate (store at room temperature)
• 2 Adhesive Plate Sealers (store at room temperature)

描述

The MEGAclear™-96 Kit is designed for the quick, high-throughput cleanup of any large-scale transcription reaction. The kit efficiently separates RNA from unincorporated NTPs, enzymes, and buffer components.

• Purifies transcription reactions in a 96-well format to produce high quality RNA free of unincorporated NTPs, enzymes, and buffer components
• Quick protocol—purify RNA in less than 20 minutes
• Can be adapted to robotic platforms

The MEGAclear™-96 Kit offers more consistent results and higher recovery than generic RNA isolation methods that have been adapted for RNA clean-up. The protocol is quick and easy. The sample is mixed with Binding Solution and ethanol, transferred to a 96-well filter plate, and washed twice; an elution step releases purified RNA, ready for any application requiring highly pure synthetic or in vitro-transcribed RNA. The MEGAclear-96 Kit can also be integrated with your robotic platform or used with manual methods.

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