规格
Accession Number:	NM_000740.2
Assay Entry:	Cell-based beta lactamase reporter gene
Cell Line:	CHO-K1
Cell State:	Dividing Cells
Druggable Target:	GPCRs
Gene Symbol:	M3
Population Status:	Single Cell Clone Origin
Product Line:	GeneBLAzer®
Reporter Gene:	BLA (Beta-Lactamase)
System Type:	GeneBLAzer™
Target Entry:	M3
Technique:	FRET
Detection Method:	Fluorescent

储存

Contents:
K1716 GeneBLAzer® M3-NFAT-bla CHO-K1 cells
(LiveBLAzer™-FRET B⁄G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:
• GeneBLAzer® M3-NFAT-bla CHO-K1 cells
• GeneBLAzer® M3-NFAT-bla CHO-K1 Assay Protocol
• Certificate of Analysis

Storage:
The GeneBLAzer® M3-NFAT-bla CHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.

描述

GeneBLAzer® M3-NFAT-bla CHO-K1 cells contain the human Acetylcholine (Muscarinic) Receptor 3 (M3) stably integrated into the CellSensor® NFAT-bla CHO-K1 cell line. CellSensor® NFAT-bla CHO-K1 cells (Cat. no. K1534) contain a beta-lactamase reporter gene under control of the Nuclear Factor of Activated T-cells (NFAT) response element.

The GeneBLAzer® M3-NFAT-bla CHO-K1 cells are functionally validated for Z' and EC₅₀ concentrations of Carbachol. In addition, GeneBLAzer® M3-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

The M3 receptor is localized in smooth muscle, exocrine glands, and the central nervous system. M3 has been implicated in autoimmune and neurological disorders.

For Academic pricing please login or contact us at discoverysciences@invitrogen.com.

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