规格
Vector:	pPIC
Promoter:	AOX1
Antibiotic Resistance Bacterial:	Blasticidin (BsdR)
Cloning Method:	Restriction Enzyme ⁄ MCS
Protein Tag:	His Tag (6x), c-Myc Epitope Tag

储存
Each vector kit includes 20 µg each of expression vector in A, B, & C versions, 20 µg of a lacZ (intracellular) or HSA (secreted) control vector, and an X-33 Pichia stab. Store vectors at -20°C. Store the Pichia stab at +4°C. All reagents are guaranteed stable for 6 months when properly stored.

描述

pPIC6 A, B, & C are Pichia pastoris expression vectors designed for simple selection,
high-level expression, and rapid protein purification and detection. The pPIC6 A,
B, & C vectors are designed for high-level secreted expression. Both sets of vectors
have the following features:
Blasticidin resistance for direct selection of multi-copy integrants
Inducible AOX1 promoter for high-level expression in Pichia pastoris
C-terminal c-myc epitope for convenient detection with an Anti-myc Antibody
C-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating
resin and detection with an Anti-His(C-term) Antibody
pPIC6 also features:
An É -factor secretion signal for efficient transport of proteins to the medium

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