规格
Cloning Method:	Gateway®
Key Functions:	Viral Expression
Product Line:	Gateway®, ViraPower™
Constitutive or Inducible System:	Constitutive
Delivery Type:	Adenoviral
Promoter:	CMV
Vector:	pDEST
Protein Tag:	V5 Epitope Tag

储存
• pAd-CMV⁄V5-DEST™ Vector 150 ng⁄µl in TEBuffer, pH 8.0. 40 µl (Store at -20°C) • pAd⁄CMV⁄V5-GW⁄lacZ control plasmid 1 µg⁄µl in TE Buffer, pH 8.0. 10 µl (Store at -20°C)

描述

The pAd⁄CMV⁄V5-DEST™ Gateway® Vector Kit contains the Gateway®-adapted ViraPower™ adenoviral expression vector, pAd⁄CMV⁄V5-DEST™ vector for easy recombination-based cloning and adenoviral-based, transient expression of a target gene in dividing and non-dividing mammalian cells. The vector allows generation of an adenovirus containing the target gene where constitutive, high-level expression is driven by the CMV promoter.

Advantages
• High efficiency and rapid recombination cloning
• Produces high titer adenoviral stocks
• Efficient delivery of the gene to dividing and non-dividing mammalian cells in vitro or in vivo
• Produces replication-incompetent virus for enhanced biosafety of the system
• Amenable for use in high-throughput applications

Key Features
• Gateway® Technology for efficient and rapid cloning
• CMV promoter for high-level constitutive expression of gene of interest
• Human Ad5 sequences (ΔE3) and Viral Inverted Terminal Repeats (ITRs) for packaging of the expression construct into virions
• Herpes Simplex Virus thymidine kinase (TK) polyadenylation sequence for efficient transcription termination and polyadenylation of mRNA
• V5 epitope for detection of recombinant protein
• Ampicillin selection marker

Kit Includes
• pAd⁄CMV⁄V5-DEST™ Gateway® Vector
• pAd⁄CMV⁄V5-GW⁄lacZ control plasmid

For research use only. Not intended for any therapeutic or diagnostic use.

长度(mm)
宽度(mm)
高度(mm)
重量(kg)