规格
Accession Number:	NM_005855.2
Assay Entry:	Cell-based beta lactamase reporter gene
Cell Line:	FreeStyle™ 293-F Cells
Cell State:	Dividing Cells
Druggable Target:	GPCRs
Gene Symbol:	CALCRL:RAMP1
Population Status:	Single Cell Clone Origin
Product Line:	Freestyle™, GeneBLAzer®
Reporter Gene:	BLA (Beta-Lactamase)
System Type:	GeneBLAzer™
Target Entry:	CALCRL:RAMP1
Detection Method:	Fluorescent

储存

(LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).

Includes:
• GeneBLAzer® CALCRL:RAMP1-CRE-bla Freestyle™ 293F cells
• GeneBLAzer® CALCRL:RAMP1-CRE-bla Freestyle™ 293F Assay Protocol
• Certificate of Analysis

The GeneBLAzer® CALCRL:RAMP1-CRE-bla Freestyle™ 293F cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.

描述

GeneBLAzer® CALCRL:RAMP1-CRE-bla FreeStyle™ 293F cells contain the human Calcitonin Gene Related Peptide Receptor (CALCRL:RAMP1), (Accession # NM_005795.3 NM_005855.2) stably integrated into the CellSensor® CRE-bla Freestyle™ 293F cell line. CellSensor® CRE-bla Freestyle™ 293F cells (Cat # K1130) contain a beta-lactamase (bla) reporter gene under control of the cAMP response element (CRE).

CALCRL:RAMP1-CRE-bla FreeStyle™ 293F cells are functionally validated for Z'-factor and EC50 concentrations of Alpha-CGRP. In addition, CALCRL:RAMP1-CRE-bla FreeStyle™ 293F cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section.

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