- 商品介绍
- 规格参数
- 包装参数
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规格 |
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| Chemical Reactivity: | Amine |
| Description: | Pierce NHS-Fluorescein |
| Excitation/Emission: | 494⁄518 |
| Label Type: | Classic Dyes |
| Label or Dye: | Fluorescein (FITC), FITC (Fluorescein) |
| Product Line: | Pierce™ |
| Reactive Moiety: | Active Ester, Succinimidyl Ester, NHS Ester |
| Quantity: | 100mg |
| Solubility: | DMF (Dimethylformamide) |
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储存 |
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| Store desiccated at -20°C, protect from moisture |
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描述 |
Thermo Scientific Fluorescein-5-Maleimide is a high-performance derivative of fluorescein dye, activated for easy and reliable labeling of antibodies, proteins and other molecules for use as fluorescent probes. Fluorescein-5-Maleimide is a sulfhydryl-reactive derivative of fluorescein that labels proteins and other molecules having free thiols (cysteine side chains)
Properties of Fluorescein-5-Maleimide:
• Alternative names: 5-MF, 5-maleimido-fluorescein
• Chemical name: 1H-Pyrrole-2,5-dione, 1-(3',6'-dihydroxy-3-oxospiro (isobenzofuran-1(3H),9'-(9H)xanthen-5-yl)-
• Molecular weight: 427.36±3
• Excitation source: 488 nm spectral line, argon-ion laser
• Excitation wavelength: 494 nm
• Emission wavelength: 518 nm
• Extinction coefficient: > 80,000 M-1cm-1
• CAS #: 75350-46-8
• Solubility: Soluble in DMF or DMSO
• Reactive groups: Maleimide, reacts with sulfhydryls at pH 6.5 to 7.5
Applications:
• Label antibodies for use as immunofluorescent probes
• Label oligonucleotides for hybridization probes
• Detect proteins in gels and on Western blots
Related Products
5-IAF (5-iodoacetamido-fluorescein)
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