规格
Crosslinker Type:	Heterobifunctional
Labeling Method:	Chemical Labeling
Product Line:	Pierce™
Reactive Moiety:	Amine, Azide, NHS Ester
Spacer:	Short
Description:	Pierce NHS-Azide
Solubility:	DMSO (Dimethylsulfoxide), DMF (Dimethylformamide)

储存
Upon receipt store at -20°C protected from light and moisture. Product is shipped at ambient temperature.

描述

Thermo Scientific NHS-PEG4-Azide is an amine-reactive compound that can be used to derivatize primary amines of proteins or amine-coated polymer surfaces for ligation to phosphine-modified molecules.

This NHS-ester compound reacts to form covalent bonds with primary amines (e.g., side chain of lysine residues or aminosilane-coated surfaces). The azide (N3) group reacts with phosphine-labeled molecules by a mechanism known as Staudinger chemistry, enabling efficient and specific conjugation of derivatized molecules in biological samples.

Features of NHS-PEG4-Azide:

• Soluble—reagent easily dissolves in water-miscible solvents for subsequent dilution in aqueous reaction mixtures with cell lysates and other biological samples
• Compatible—reaction chemistry occurs effectively in simple buffer conditions; requires no accessory reagents such as copper or reducing agents
• Specific—NHS esters are specific for covalent attachment to primary amines (side chain of lysine or N-terminus of polypeptides)
• Chemoselective—azide and phosphine groups do not react or interfere with components of biological samples (bioorthogonal) but conjugate to one another with high efficiency

Related Products
NHS-Azide
NHS-Phosphine

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