规格
Cleavage:	EK (Enterokinase) Recognition Site
Cloning Method:	TOPO®-TA
Key Functions:	Constitutive Expression
Product Line:	TOPO®, pcDNA™
Constitutive or Inducible System:	Constitutive
Delivery Type:	Transfection
Promoter:	CMV
Selection Agent (Eukaryotic):	Zeocin™
Vector:	pcDNA
Protein Tag:	His Tag (6x), Xpress Epitope Tag

储存

The pcDNA™4/HisMax A, B, & C vectors, 20 µg each, are supercoiled and provided lyophilized. 20 µg of pcDNA™4/HisMax/lacZ control plasmid is also provided. Store vectors at -20°C. The pcDNA™4/HisMax TOPO®TA Expression Kit contains two boxes. The TOPO® TA box contains 200 ng of linearized and topoisomerase I-activated pcDNA4/HisMax-TOPO® vector, dNTPs, control PCR template and primers, forward and reverse primers for sequencing and PCR screening, and a lacZ expression control plasmid.
Store this box at -20°C. The One Shot® box contains transformation reagents including twenty-one 50-µl aliquots of TOP10 E. coli, S.O.C. medium, and a pUC19 supercoiled control plasmid. The One Shot® box should be stored at -80°C. Guaranteed stable for 6 months when properly stored.

描述

pcDNA™4/HisMax is specifically designed to maximize protein expression in a variety of mammalian cells. The vector contains the QBI SP163 translational enhancer to increase expression levels two- to five-fold above those seen with the promoter alone (Figure 1). In addition to SP163-enhanced expression, pcDNA™4/HisMax includes a cleavable N-terminal Xpress™ tag for rapid detection of recombinant protein with an Anti-Xpress™ Antibody (Figure 2). pcDNA™4/HisMax is available TOPO® Cloning-ready for five-minute cloning of Taqamplified PCR products.

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