规格
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Final Product Type:
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BAC DNA |
For Use With (Application):
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Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, PCR, In Vitro Transcription |
Purification Target:
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BAC DNA, Plasmid DNA |
Sample Type:
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Bacterial Culture |
Isolation Technology:
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Anion Exchange Resin |
High-throughput Compatibility:
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Not High-throughput Compatible (Manual) |
Shipping Condition:
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Room Temperature |
Product Line:
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PureLink™ |
储存
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The PureLink® HiPure BAC Buffer Kit includes Resuspension Buffer (R3) (250 ml), Lysis Buffer (L7) (250 ml), Precipitation Buffer (N3) (250 ml), and RNase A (20 mg/ml) (5 ml). All components of the PureLink® HiPure BAC Buffer Kit are shipped at room temperature. Store all components at room temperature upon receipt. |
The PureLink® HiPure BAC Buffer Kit provides additional buffers and reagents for performing BAC (Bacterial Artificial Chromosome) DNA isolation using the PureLink® HiPure Plasmid Kits. The PureLink® HiPure Plasmid Kits utilize a proprietary anion-exchange resin which allows you to purify high-quality BAC DNA from E. coli cultures. BAC DNA requires the use of large bacterial culture volumes to obtain a reasonable DNA yield. The additional buffers and reagents needed for working with larger bacterial cultures are provided in this Buffer Kit. From a 100 ml culture, you can expect to purify ~40 µg of BAC DNA. The PureLink® HiPure BAC Buffer Kit provides reagents sufficient for five BAC Maxipreps, or 25 BAC Midipreps, or 100 BAC Minipreps.