规格
|
Key Functions:
|
Reporter Assays, Constitutive Expression |
Shipping Condition:
|
Approved for shipment on Wet or Dry Ice |
Constitutive or Inducible System:
|
Constitutive |
Delivery Type:
|
Transfection |
Promoter:
|
CMV |
Reporter Gene:
|
Beta-Gal (lacZ) |
Selection Agent (Eukaryotic):
|
None |
Vector:
|
pCMV |
Protein Tag:
|
Untagged |
储存
|
Store at +4°C. Store at -20°C long term. |
Plasmid pCMV·SPORT-bgal is used as a positive control for
monitoring expression in eukaryotic cells. The plasmid contains the
reporter gene b-galactosidase (b-gal) from E. coli cloned as a Not I
fragment into plasmid pCMV·SPORT1. The plasmid also contains
a CMV promoter upstream of the b-gal gene, followed by the SV40
t-intron and polyadenylation signal. The b-lactamase gene allows
selection for ampicillin resistance in E. coli.