TQMN,REV TRANSCRIPTION RGNTS/ MNL 402876，N8080234，Applied Biosystems 供应商、价格、厂家、报价、规格参数

产品介绍 Product Description

规格
Final Product Type:	cDNA (First-Strand)
For Use With (Application):	Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR)
Sample Type:	RNA
Quantity:	200 reactions
Size (Final Product):	5 kb or less
Optimal Reaction Temperature:	42° C
Reaction Format:	SuperMix or Master Mix, Separate Components
Reverse Transcriptase:	MultiScribe™
Shipping Condition:	Dry Ice
Product Line:	TaqMan™

储存

Contents:
• 1 × 100 µL of MultiScribe™ Reverse Transcriptase (50U/µL)
• 1 × 100 µL of RNase Inhibitor (20U/µL)
• 1 × 1 mL of dNTP Mixture (2.5 mM each dNTP)
• 1 × 100 µL of Oligo d(T)16 (50 µM)
• 1 × 100 µL of Random Hexamers (50 µM)
• 1 × 1.5 mL of 10X RT Buffer
• 1 × 1.5 mL of MgCl₂ Solution

Reagents should be stored between -15°C and -25°C.

A note about the size: This kit contains enough reagents for 200 reverse transcriptase reactions when the reactions are set up at 10 µL each.

描述

TaqMan® Reverse Transcription Reagents provide the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, oligo (dT)₁₆, and reverse primers are included for cDNA synthesis flexibility. Advantages of TaqMan® Reverse Transcription Reagents:

• Contain MultiScribe® Reverse Transcriptase, a recombinant moloney murine leukemia virus reverse transcriptase (MMLV RT)
• Random hexamers and oligo (dT)₁₆ included for flexibility
• Individual components provide versatility in assay set-up
• Compatible with two-step RT-PCR reactions

Using TaqMan® Reverse Transcription Reagents
TaqMan® Reverse Transcription Reagents provide all the necessary components to perform the reverse transcription of RNA to cDNA. For two-step RT-PCR, random hexamers, oligo (dT)₁₆, and sequence-specific reverse primers can all be used. The choice of primers for reverse transcription is best made after experimentally evaluating all three priming systems.

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