SS INDIRECT CDNA LABELING MOD 30 RXNS，L101403，Invitrogen 供应商、价格、厂家、报价、规格参数

产品介绍 Product Description

规格
Final Product Type:	cDNA (Labeled)
Includes Label or Dye:	No
Labeling Method:	Indirect Labeling
Labeling Target:	cDNA
Label or Dye:	Alexa Fluor™ 555, Alexa Fluor™ 647, Cy™3, Cy™5
Product Line:	SuperScript™
Sample Type:	RNA
Detection Method:	Fluorescent

储存

The SuperScript® Indirect cDNA Labeling Module includes SuperScript® III Reverse Transcriptase, oligo(dT)_20- VN (2.5 µg/µl), random primers, control RNA ladder (0.5 g/µl), 5X first-strand buffer, 0.1 M DTT, 10 mM dNTP mixture, RNaseOUT™ Recombinant Ribonuclease Inhibitor (40 U/µl), DEPC-treated water, DMSO (Labeling Grade), 2X coupling buffer, 3M NaAc, pH 5.2, and glycogen (20 mg/ml). The SuperScript® Indirect cDNA Labeling System includes all of the components listed for the Labeling Module, plus S.N.A.P.™ column, collection tubes, loading buffer, wash buffer, amber collection tubes. Store all Labeling Module components at -20°C. Store S.N.A.P.™ columns, collection tubes, loading buffer, wash buffer, and amber collection tubes at room temperature. Guaranteed stable for 6 months when properly stored.

描述

The SuperScript® Indirect cDNA Labeling System is an array labeling kit based on proven methods of indirect cDNA labeling. The optimized system provides:

• SuperScript® III RT for generating high cDNA yields
• A proprietary nucleotide mixture to increase signal intensity
• A convenient kit format that saves valuable time

In this indirect labeling method, mRNA or total RNA is reverse transcribed using SuperScript® III RT, incorporating amino-modified dUTP and dATP into the synthesized cDNA. The template RNA is then degraded by base hydrolysis, and the reaction is neutralized with acid. The amino-modified cDNA is then purified to remove unincorporated nucleotides, primers, and buffers. In the second step, the modified cDNA is coupled with the active form of a fluorescent dye. The fluorescently labeled cDNA is purified with a S.N.A.P.™ spin column to remove any unreacted dye. The resulting fluorescently labeled cDNA is ready for hybridization to microarrays (Figure 1).

The SuperScript® Indirect cDNA Labeling System provides a complete system that includes both the core kit reagents (except dyes) and a purification module. The SuperScript® Indirect cDNA Labeling Module includes just the core kit reagents. Both provide the flexibility to use fluorescent nucleotides of your choice.

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