PBAD/MYC-HIS A,B,C KIT 1 KIT，V44001，Invitrogen

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订货号： 7CA3221

品牌型号： Invitrogen V44001

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最小订货量： 1套

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商品介绍
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产品介绍 Product Description

规格
Antibiotic Resistance Bacterial:	Ampicillin (AmpR)
Cleavage:	EK (Enterokinase) Recognition Site
Cloning Method:	Restriction Enzyme ⁄ MCS
Constitutive or Inducible System:	Inducible
Inducing Agent:	Arabinose
Promoter:	araBAD
Selection Agent (Eukaryotic):	None
Vector:	pBAD
Protein Tag:	c-Myc Epitope Tag

储存
The pBAD/Myc-His Kit includes 20 µg of pBAD/Myc-His A, B, & C vector, 20 µg of pBAD/Myc-His/lacZ, 20% L-arabinose, LMG194 and TOP10 E. coli stabs. Vectors and 20% L-arabinose should be stored at -20°C. Store LMG194 and TOP10 stabs at 2 - 8°C. All components are guaranteed stable for 6 months when properly stored.

描述

The pBAD/Myc-His Kit provides all of the necessary reagents to express your protein in a tightly regulated fashion. The pBAD/Myc-His vector expresses native proteins or fusion proteins with a C-terminal tag. The vector provides:

• The araBAD promoter for tightly regulated expression
• Translation initiation signals optimized for E. coliexpression
• C-terminal polyhistidine (6xHis) tag for purification with nickel-chelating resin or detection with an Anti-His(C-term) Antibody
• C-terminal c-myc epitope for detection and analysis with an Anti-myc Antibody

Three vectors are provided (A, B, and C). Each has the C-terminal tag in a different reading frame relative to the multiple cloning site to simplify in-frame cloning of your gene.

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