- 商品介绍
- 规格参数
- 包装参数
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规格 |
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| Assay Entry: | Biochemical binding/kinetic |
| Kinase Group: | Serine/Threonine Kinase |
| Species: | Human |
| Target Entry: | ULK1 |
| Molecular Weight: | 96.1 kDa |
| Quantity: | 10μg |
| Gene Alias: | ATG1, UNC51, Unc51.1, FLJ38455 |
| Validated Application: | Kinase Assay |
| Protein Length: | aa 1-649 |
| Protein Tag: | GST Tag |
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储存 |
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Storage Buffer: 50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.25 mM DTT, 10 mM glutathione, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol Store at -68 to -85°C. Aliquot at first use to avoid multiple freeze-thaws. |
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描述 |
ULK1 is a serine/threonine protein kinase that plays critical role during initial stages of autophagy which is a vital response to nutrient starvation. The conserved C-terminal domain (CTD) of ULK1 controls the regulatory function and localization of the protein. Knockdown of ULK1 inhibits the autophagic response as well as inhibiting rapamycin-induced autophagy consistent with a role downstream of mTOR. ULK1 forms a complex with FIP200 and ATG13 and this complex is essential for starvation-induced autophagy. Both FIP200 and ATG13 are critical for correct localization of ULK1 to the pre-autophagosome and stability of ULK1 protein. ULK1 is phosphorylated by the mTOR pathway in a nutrient starvation-regulated manner. Recombinant human ULK1 (1-649) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
• Validated in LanthaScreen® Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use
Related Product:
LanthaScreen® Eu Kinase Binding Assay
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¥ 749.00
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¥ 66743.99
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¥ 145427.01
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¥ 46052.83
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