PepTivator® JCV LT, premium grade，130-131-541，美天旎

Stimulate T cells reactive to the large T (LT) antigen of the JC virus (JCV) using PepTivator Peptide Pools. PepTivators are pools of lyophilized peptides, consisting mainly of 15-mer sequences with 11 amino acids overlap. PepTivator^® JCV LT is a pool of lyophilized peptides, covering the sequence of the JCV LT protein (UniProtKB Acc. no. P03072-1).

In vitro stimulation of antigen-specific T cells with PepTivator Peptide Pools causes the secretion of effector cytokines and the up-regulation of activation markers, which then allow the detection and isolation of antigen-specific T cells.

The PepTivator is available in two different quality grades. The research-grade product has an average purity of 70%, whereas peptides of the premium-grade product are individually purified via HPLC having each a purity of >80%. For translational applications involving the MACS GMP PepTivator JCV LT, please choose the premium-grade product.

The John Cunningham virus (JCV) is a widespread human polyomavirus with high homology to BK polyomavirus (BKV). Primary JCV infection results in asymptomatic latent persistence of the virus in the gastrointestinal tract and the kidney. Immunodeficiency or immunosuppression allows virus reactivation, which can cause, e.g., a fatal progressive multifocal leukoencephalopathy. The large T antigen (LT) is a protein expressed early in the lytic cycle, initiating viral replication. It has been shown to be a target of T cell immunity.

PepTivator JCV LT – research grade has been specifically developed for efficient in vitro stimulation of JCV-specific T cells. Peptides of 15 amino acids in length and 11 amino acids overlap represent an optimized solution for stimulating both CD4⁺ and CD8⁺ T cells in various applications, including:

• Detection and analysis of JCV-LT–specific effector/memory T cells in PBMCs by MACS® Cytokine Secretion Assays, intracellular cytokine staining, or other technologies.
• Isolation of viable JCV-LT–specific CD4⁺ T cells with the CD154 MicroBead Kit, or of CD4⁺ and CD8⁺ T cells using the CD137 MicroBead Kit or MACS Cytokine Secretion Assay – Cell Enrichment and Detection Kits. Subsequently, cells can be expanded for generation of T cell lines.
• Generation of JCV-LT–specific effector/memory T cells from naive T cell populations.
• Pulsing of antigen-presenting cells.