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CALCR-CRE-BLA FS 293F CELLS COMBO,K1480,Invitrogen

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订货号 6ZL9415
品牌型号 Invitrogen K1480
货期 询货期
最小订货量 1套
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产品介绍 Product Description
规格
Accession Number: NM_001742.2
Assay Entry: Cell-based beta lactamase reporter gene
Cell Line: FreeStyle™ 293-F Cells
Cell State: Dividing Cells
Druggable Target: GPCRs
Gene Symbol: CALCR2
Population Status: Single Cell Clone Origin
Product Line: GeneBLAzer®
Reporter Gene: BLA (Beta-Lactamase)
System Type: GeneBLAzer™
Target Entry: CALCR2
Detection Method: Fluorescent
储存
(LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:
o GeneBLAzer® CALCR-CRE-bla Freestyle™ 293F cells
o GeneBLAzer® CALCR-CRE-bla Freestyle™ 293F Assay Protocol
o Certificate of Analysis
The GeneBLAzer® CALCR-CRE-bla Freestyle™ 293F cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
描述

The GeneBLAzer® CALCR-CRE-bla Freestyle293 cells contain the CALCR receptor isoform (CTR2/CT(a)/CTRI1-), accession # NM_001742.2, stably integrated into the CellSensor® CRE-bla Freestyle293 cell line. The CellSensor® CRE-bla Freestyle293 cells contain a beta-lactamase (bla) reporter gene under control of the cAMP (CRE) response element.

CALCR-CRE-bla Freestyle293 cells have been tested for variable assay conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time and functionally validated for Z' and EC50 concentrations of calcitonin. Additional testing data using alternate stimuli are also provided. These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section.

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