规格
Agent:	Puromycin
Formula Weight:	544.43 g⁄mole
Product Line:	Gibco®
Reagent Type:	Antibiotic (Selective)
Shipping Condition:	Dry Ice
Validated Application:	Eukaryotic Selection⁄Stable Cell Line Generation
Form:	Liquid
Concentration:	10 mg⁄ml, 10 mg⁄mL, 10 mg⁄ml
Quantity:	1 bottle
Sterility:	Sterile

储存
Storage conditions: -5 to -20°C Shipping conditions: Frozen Shelf life: 12 months from date of manufacture

描述

Puromycin Dihydrochloride is an aminonucleoside antibiotic produced by Streptomyces alboniger. Puromycin works by inhibiting peptidyl transfer on both prokaryotic and eukaryotic ribosomes; resistance is conferred by the expression of the pac gene.

Puromycin is widely used in cell biology as a selection antibiotic agent in mammalian cell culture systems. The recommended working concentration ranges from 0.2–5.0 µg/mL, although it can be toxic to eukaryotic cells at concentrations as low as 1 µg/mL. Gibco® Puromycin Dihydrochloride is supplied at 10 mg/mL in 20 mM HEPES buffer (pH 6.2–6.8) in 10 vials each containing 1 mL.

Other Choices and More Information
We offer a wide range of antibiotics and antimycotics in both powder and liquid formats.

See the complete list, or find products for:
• Contamination control
• Eukaryotic and bacterial selection

See recommendations for working concentrations for selection antibiotics.

Learn more about the use of antibiotics and antimycotics in cell culture, and review guidelines for decontaminating cultures.

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