规格
Detection Location:	In-Gel Detection
Detection Method:	Colorimetric
Label or Dye:	SilverQuest™ Silver Stain
Product Line:	SilverQuest™
Target Molecule:	Protein

储存
This kit contains sufficient reagents to stain 25 minigels Contains: 250 mL Sensitizer 25 mL Stainer 250 mL Developer 2 mL Developer Enhancer 250 mL Stopper solution 60 mL Destainer A 60 mL Destainer B Store kit at room temperature (15°C to 30°C). Stable for 6 months.

描述

The SilverQuest Silver Staining Kit provides a rapid and easy method to silver stain proteins and DNA in polyacrylamide gels. It is specifically designed to provide sensitive silver staining compatible with mass spectrometry analysis.

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Features of this kit:
• Compatible—staining compatible with mass spectrometry analysis
• Protocol—protein bands (0.3 ng of protein) can be visualized within an hour or for added speed a microwave procedure can be completed in ~30 minutes
• Color-coded solutions—color-coded solutions for easy staining protocol

Mass spectrometry-compatible reagents
The SilverQuest Silver Staining Kit has been specifically designed to make protein detection compatible with mass spectrometry analysis. The SilverQuest reagent set contains a sensitizer that contains no glutaraldehyde or formaldehyde, so it leaves lysine and arginine side-chains unmodified. Additionally, the SilverQuest destaining solution effectively removes silver from protein bands in polyacrylamide gels. Peptides remain as efficient targets for in-gel trypsin digestion and gel extraction and yield better coverage during mass spectrometry analysis.

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