规格
Detection Location:	In-Cell Detection, In-Gel Detection
Detection Method:	Fluorescent
For Use With (Equipment):	Confocal Microscope, Fluorescence Microscope, Flow Cytometer, High Content Instrument
Form:	Solid
Label Type:	Unconjugated
Label or Dye:	Unconjugated
Product Line:	Click-iT™
Shipping Condition:	Dry Ice
Color:	None
Solubility:	DMSO (Dimethylsulfoxide)

储存
Store at ≤ -20°C. Desiccation recommended. Protect material from long-term exposure to light; may be exposed to light for short periods of time

描述

Conjugates prepared with this thiol-reactive maleimide DIBO alkyne, can be detected with an azide-containing molecule in a copper-free click chemistry reaction. Click chemistry describes a class of chemical reactions that use bio-orthogonal or biologically unique moities to label and detect a molecule of interest using a two-step procedure. The two-step reaction procedure involves triazole formation of an azide and an alkyne. Click reactions have several characteristics: the reaction between the detection moieties is efficient; no extreme temperatures or solvents are required; the reaction product is stable; the components of the reaction are bioinert; and perhaps most importantly, no side reactions occur, and with the elimination of copper, no detectable damage to cells or proteins. Unlike traditional chemical reactions utilizing succinimidyl esters or maleimides that target amines and sulfhydryls – functional groups that are not unique – click chemistry-labeled molecules can be applied to complex biological samples and be detected with unprecedented sensitivity due to extremely low background.

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