规格
Bacterial or Yeast Strain:	MaV203
Blue/White Screening:	No
Competent Cell Type:	Chemically Competent
F'episome (to make ssDNA):	Lacks F' Episome
High-throughput Compatibility:	Not High-throughput Compatible (Manual)
Improves Plasmid Quality:	No
Product Line:	ProQuest™
Shipping Condition:	Dry Ice
Species:	S. cerevisiae
Transformation Efficiency:	> 1x10^6
Transformation Efficiency Level:	Subcloning Efficiency (10^6-10^7 cfu⁄µg)
Format:	Tube(s)

储存
Store in ultra-cold freezer (-68 to -85°C).

描述

MaV203 Competent Yeast Cells are designed for use with the ProQuest™ Two-Hybrid System (Figure 1). S. cerevisiae strain MaV203 contains deletions in the endogenous GAL4 and GAL80 genes for use with GAL4-based two-hybrid systems. The strain also has leu2 and trp1 mutations for selection of ProQuest™ bait and prey vectors. MaV203 contains three GAL4 inducible reporter genes, providing four phenotypes, for easier identification of true interactors (Figure 2). The MaV203 competent cells feature:

• Transformation (250 µl of cells) efficiency yields >1 x 10⁶ colonies
• Control DNA pMAB12 to confirm transformation efficiency and the preparation of selection media
• Pre-qualified PEG/Lithium Acetate to maximize number of trans-formants
• Competent cells formatted with carrier DNA already included

长度(mm)
宽度(mm)
高度(mm)
重量(kg)