- 商品介绍
- 规格参数
- 包装参数
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规格 |
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| Antibiotic Resistance Bacterial: | Ampicillin (AmpR) |
| Cloning Method: | Restriction Enzyme ⁄ MCS |
| Constitutive or Inducible System: | Inducible |
| Inducing Agent: | Arabinose |
| Promoter: | araBAD |
| Selection Agent (Eukaryotic): | None |
| Vector: | pBAD |
| Protein Tag: | c-Myc Epitope Tag |
|
储存 |
|
| The pBAD/gIII Kit includes 20 µg each of pBAD/gIII A, B, & C, 20 µg of pBAD/gIII/calmodulin, 1 ml 20% L-arabinose, LMG194 and TOP10 E. coli stabs. Store stabs at 2 - 8°C. Store all other components at -20°C. All components are guaranteed stable for 6 months when properly stored. |
|
描述 |
The pBAD/gIII Kit provides all of the necessary reagents to express recombinant proteins under the tight regulation of the araBAD promoter. To simplify purification, the vector pBAD/gIII encodes a leader peptide which directs the recombinant protein into the
periplasmic space. The vector provides:
• The araBAD promoter for tightly regulated expression
• Translation initiation signals for optimized E. coliexpression
• Leader peptide from the bacteriophage fd gene III protein (gIII) for secreted expression
• C-terminal polyhistidine (6xHis) tag for purification with nickel-chelating resin
• C-terminal c-myc epitope for detection and analysis with an Anti-myc Antibody
A set of three vectors is provided (A, B, and C). Each has the C-terminal tag in a different reading frame relative to the multiple cloning site to simplify in-frame cloning of your gene.
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¥ 2250.00
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