规格
Cloning Method:	Restriction Enzyme ⁄ MCS
Key Functions:	Constitutive Expression
Constitutive or Inducible System:	Constitutive
Delivery Type:	Transfection
Promoter:	CMV
Selection Agent (Eukaryotic):	Zeocin™
Vector:	pCMV
Protein Tag:	Untagged

储存
20 µg ZeoCassette™ vector is supplied supercoiled and lyophilized. Store at -20°C. Zeocin™ should be protected from light. Vectors are guaranteed stable for 6 months when properly stored.

描述

The ZeoCassette™ vectors allow you to incorporate the Zeocin™ resistance gene into any vector. Each vector carries a ZeoCassette™ which contains the Zeocin™ resistance marker driven by a strong constitutive promoter (Table 1). Zeocin™ is a highly effective selection agent that kills a wide variety of cell types including bacterial, yeast, plant, and mammalian cells. The ZeoCassette™ vectors can be used in a variety of ways:

• Insert your sequence of interest into one of the ZeoCassette™ vectors to create your own Zeocin™-resistant vector
• Incorporate one of the ZeoCassettes™ into a vector that youve already constructed
• Clone your promoter of choice upstream of the EM-7 promoter in pEM7/Zeo and use Zeocin™ selection in your organism of choice

Each ZeoCassette™ is flanked by large multiple cloning sites. This allows removal of the ZeoCassette™ for incorporation into your vector of choice. In addition, the Zeocin™ resistance gene in pSV40/Zeo, pCMV/Zeo, and pTEF1/Zeo is also driven by the bacterial EM-7 promoter. This allows you to use Zeocin™ to select E. colitransformants that contain the ZeoCassette™ and eliminates the need to screen colonies by restriction enzyme analysis.

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