规格
Accession Number:	NM_000738
Assay Entry:	Cell-based beta-arrestin recruitment
Cell Line:	U2OS
Cell State:	Dividing Cells
Cytoplasmic Tail:	Native Tail
Druggable Target:	GPCRs
Human Protein Class:	G-Protein Coupled Receptor (GPCR)
Population Status:	Single Cell Clone Origin
Product Line:	Tango™
Readout:	End Point
Reporter Gene:	BLA (Beta-Lactamase)
System Type:	Tango™
Target Entry:	M1
Technique:	FRET
Detection Method:	Fluorescent

储存
Tango™ cell lines are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.

描述

Tango™ M1-bla U2OS cells contain the human muscarinic M1 receptor (CHRM1) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin⁄TEV protease fusion protein and the beta-lactamase reporter gene under the control of a UAS response element. The Tango™ M1-bla U2OS cells have been functionally validated for Z’ factor and EC50 concentrations of oxotremorine M. In addition, Tango™ M1-bla U2OS cells have been tested for assay performance under variable conditions.

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