规格
Assay:	Phosphatase Assay
Excitation/Emission:	358⁄452
Product Line:	EnzChek®, RediPlate™
Shipping Condition:	Room Temperature
Substrate:	DiFMUP
Substrate Properties:	Chemical Substrate
Substrate Type:	Phosphatase Substrate
Target Enzyme:	Serine⁄Threonine Phosphatases
Technique:	Fluorescence Intensity
Detection Method:	Fluorescent
For Use With (Equipment):	Microplate Reader
Label or Dye:	DiFMU

储存
Store in freezer (-5 to -30°C) and protect from light.

描述

The RediPlate™ 96 EnzChek® Serine/Threonine Phosphatase Assay Kit provides a fast, simple and direct fluorescence-based assay for detecting serine/threonine phosphatases and their corresponding modulators and inhibitors. Unlike other microplate assays, this kit provides the necessary reagents predispensed into a 96-well microplate. Simply reconstitute the fluorogenic substrate in the assay wells with buffer, add the desired sample to the wells, incubate and then quantitate the fluorescence in any standard fluorescence-based microplate reader. Inhibitors are included in each assay well to ensure that the assay is selective for Ser/Thr PPases- other phosphatases, including tyrosine phosphatase do not significantly react with the substrate. Additional advantages of this assay include compatibility with non-ionic detergents and insensitivity to free phosphate.

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