规格
|
Cloning Method:
|
Restriction Enzyme ⁄ MCS |
Key Functions:
|
Constitutive Expression |
Constitutive or Inducible System:
|
Constitutive |
Delivery Type:
|
Transfection |
Promoter:
|
EF-1α |
Selection Agent (Eukaryotic):
|
Blasticidin |
Vector:
|
pEF |
Protein Tag:
|
His Tag (6x), c-Myc Epitope Tag |
储存
|
Vectors (and appropriate expression control) are supplied supercoiled and lyophilized. Stored at -20°C. All vectors are guaranteed stable for 6 months when properly stored. |
All pEF or pUB vectors contain a strong promoter for high-level expression in mammalian cells, a choice of selection marker for generating stable cell lines, and an epitope tag for easy detection with a monoclonal antibody and rapid purification on nickel-chelating resin. Each vector is available in three reading frames to simplify cloning in-frame with the fusion tag.