DYLIGHT 680-4XPEG, NHS ESTER, 1 MG，46601，赛默飞世尔

Thermo Scientific DyLight 680-4xPEG Amine-Reactive Dye is a derivative of our high-performance DyLight 680 Dye that can be used to fluorescently label antibodies and other proteins.

The DyLight 680-4xPEG dye contains 4 polyethylene glycol (PEG) chains that are non-cytotoxic, enhance fluorescence and reduce nonspecific binding of conjugates made with them. Conjugates made with DyLight 680-4xPEG Dye can be used as molecular probes for cellular imaging and other fluorescence detection methods. The far-red to NIR fluorescence properties of DyLight 680-4xPEG Dye make it especially useful in a variety of biological, chemical, and pharmaceutical applications, including in vivo imaging. The PEG chains also improve solubility of the dyes and labeled molecules in aqueous solution, aid in cell permeability and improve tissue retention.

Features of the DyLight 680-4xPEG NHS Ester:

• High fluorescence intensity—significantly brighter fluorescence than Alexa Fluor™ 680
• PEGylated—improves solubility in aqueous solution, aids in cell permeability and improves tissue retention in in vivo applications

Applications:
• Fluorescence microscopy
• In vivo or ex vivo imaging
• Cell-based assays
• Flow cytometry/fluorescence-activated cell sorting (FACS)

DyLight 680-4xPEG Amine-Reactive Dye is activated with an N-hydroxysuccinimide (NHS) ester moiety to react with exposed N-terminal α-amino groups or the ε-amino groups of lysine residues to form stable amide bonds. Learn more about NHS ester chemistry.

Typical labeling reactions require the dye to first be dissolved in anhydrous dimethyl formamide (DMF) or another suitable organic solvent before adding a specific molar amount of dye to an amine-free buffer containing the protein to be labeled. However, the high solubility of DyLight Fluors permits protein solutions to be added directly to the labeling reagent. This feature allows DyLight 680-4xPEG Amine-Reactive Dye to be provided in multiple formats with flexible protocols to achieve efficient degrees of labeling.

We also offer Standard and Microscale DyLight 680-4xPEG Antibody Labeling Kits for fast and efficient fluorescent labeling of antibodies. The standard size kit contains all necessary components to perform three separate labeling reactions using 1 mg IgG or similar quantities of other proteins. The microscale kit contains all of the necessary components to perform five separate labeling reactions using 100 µg IgG. Both kit sizes include the Amine-Reactive DyLight 680-4xPEG NHS-ester in convenient single-use vials as well as purification resin and spin columns for the preparation of ready-to-use conjugate.

Related Products
DyLight™ 680-4xPEG Antibody Labeling Kit
DyLight™ 680-4xPEG Microscale Antibody Labeling Kit