规格
Final Product Type:	Total RNA, Viral RNA
For Use With (Application):	Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting
For Use With (Equipment):	MagMAX™ Express 24 and 96, Robotic Liquid Handling Systems, MagMAX™ Express-96 Standard Magnetic Particle Processor
Purification Target:	Viral DNA⁄RNA, Total RNA
Sample Type:	Liquid Samples (e.g. Serum), Viral Samples, Whole Blood (Mammalian), Milk
Format:	96-well plate
Isolation Technology:	Magnetic Bead
Yield:	0.5 µg
High-throughput Compatibility:	High-throughput Compatible, Not High-throughput Compatible (Manual), Automated Protocols
Prep Scale:	< 100 µl Blood Samples (Small-Scale)
Starting Material Amount:	Up to 50 µl whole blood
Product Line:	Ambion™, MagMAX™

储存
Store Processing Plate with Lid, Lysis/Binding Solution Concentrate, Wash Solution 1 Concentrate, Wash Solution 2 Concentrate, and Elution Buffer at room temp. Store MagMax™Turbo™DNase Buffer and RNA Binding Beads at 4°C. Store Lysis/Binding Enhancer and TURBO™DNase at -20°C.

描述

This Ambion® kit is for the rapid, high-throughput purification of pathogen RNA from mammalian whole blood, milk, and other biofluids in 96-well plates. RNA purified using the kit is ideal for RT-PCR applications. The kit uses magnetic bead–based purification and includes sufficient reagents for 96 reactions.

• Easily isolate viral or total RNA from whole blood
• Save precious time—complete the protocol in less than 1 hour
• Walk away—integrate with established robotic platforms
• Validated with human, bovine, porcine, and ovine whole blood

Benefits of MagMAX™ Magnetic Bead-Based Purification:
Magnetic beads offer many benefits compared to other technologies for isolating RNA. Beads bind RNA more efficiently than glass fiber filters, resulting in higher and more consistent RNA yields. Additionally, because filters are not used, there is no risk of filter clogging due to cellular particulates in samples. Since only a small volume of magnetic beads is needed for high- efficiency binding, the bound RNA can be eluted in just 30–50 µL of nuclease-free water, concentrating RNA from large, dilute samples.

Isolate RNA from as Little as 50 µl of Blood:
For the highest-quality RNA, use whole blood samples that have not been frozen. Approximately 300–500 ng of intact RNA can be consistently purified from 50 µl of human whole blood. Bovine, porcine, and ovine whole blood samples can also be used. Yields vary from species to species (see data). The recovered RNA is devoid of PCR inhibitors such as proteins and heme, commonly found in RNA isolated from blood.

Isolate Viral and Total RNA from Whole Blood Samples:
The MagMAX™-96 Blood RNA Isolation Kit eliminates sample preprocessing, which can cause sample mix-up and cross-contamination. The procedure is fast (<1 hour), simple, and well suited for automation. The kit can also be used to process fewer than 96 samples at one time and is compatible with either manual processing using multi-channel pipettors or processing with robotic liquid handlers. Downloadable protocols for the use of this kit with specific liquid handling systems are available at our Automation Resource. The MagMAX™-96 Blood RNA Isolation Kit can also be used for total nucleic acid isolation by omitting the DNase treatment.

Accessory Products:
Lysis/Binding Solution Concentrate (SKU#AM8500), Wash Solution 1 Concentrate (SKU#AM8504), and Wash Solution 2 Concentrate (SKU#AM8640) are components of the kit that may also be purchased separately. A 96-well magnetic ring stand is required to use this kit. The Magnetic Stand-96 (SKU#AM10027) and the 96-well Magnetic Ring Stand (SKU#AM10050) are designed for paramagnetic bead precipitation from standard 96-well U-bottom microplates. The Magnetic-Ring Stand pellets the magnetic beads in a circle shape, enabling easy removal of supernatant by a robot or multichannel pipettors. For additional processing plates, Matrix 96-Well U Bottom Polypropylene Plates (Thermo Scientific, Item# 4917) are recommended.

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