规格
Detection Method:	Primer-Probe Detection
For Use With (Equipment):	7000 System, 7300 System, 7700 System, 7900HT Fast System, 7900HT System, Eppendorf® MasterCycler® RealPlex
Includes:	10mL Resuspension Buffer; 1mL Lysis Enhancer; 2 x 1.25mL DNase I, Amplification Grade (1U/μL); 800μL 10X DNase I Buffer; 2 x 1mL 25mM EDTA; 500μL SuperScript III RT/Platinum Taq Mix; 12.5mL 2X Reaction Mix with ROX; 1mL 50mM MgSO₄; 12.5mL DEPC-treated water<; 10μL HeLa Total RNA (10ng/μL)
Quantity:	500 reactions
Sample Type:	Cells
PCR Method:	1-Step qRT-PCR
Reaction Speed:	Fast, Standard
Product Line:	CellsDirect™, Platinum®, SuperScript™

储存

• 10 ml Resuspension Buffer
• 1 ml Lysis Enhancer
• 2 × 1.25 ml DNase I, Amplification Grade (1 U/µl)
• 800 µl 10X DNase I Buffer
• 2 × 1 ml 25 mM EDTA
• 500 µl SuperScript® III RT/Platinum® Taq Mix
• 12.5 ml 2X Reaction Mix with ROX™
• 1 ml 50 mM MgSO₄
• 12.5 ml DEPC-treated water
• 10 µl HeLa Total RNA (10 ng/µl)
The kit is shipped on dry ice and should be stored at -20°C

描述

The CellsDirect™ One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step.

Features of the CellsDirect™ One-Step qRT-PCR Kit include:

• Efficiency—Offers significant time and cost savings for increased throughput
• Convenience—Enzyme mix includes both SuperScript® III Reverse Transcriptase and Platinum® Taq DNA Polymerase
• Sensitivity—No separate RNA isolation step increases sensitivity by minimizing sample loss
• Broad Detection Range—Detect targets from samples ranging from a single cell to 10,000 cells
• Versatility—Compatible with a wide variety of cell lines and samples collected by Laser Capture Microdissection (LCM)

Streamline Your Workflow
In traditional real-time quantitative RT-PCR (qRT-PCR), you first isolate RNA from cells in a time-consuming procedure that can lead to loss of material. Using the CellsDirect™ One-Step qRT-PCR Kit, you lyse the cells and add the complete lysate directly to a one-step qRT-PCR reaction with minimal handling and sample loss.

Ideal for Your qRT-PCR Application
CellsDirect™ technology is validated for gene expression studies involving limited samples, and is suitable for validation of RNAi knockdowns. CellsDirect™ One-Step qRT-PCR Kits are especially valuable for high-throughput applications by eliminating costly, time-consuming RNA purification steps (Figure 1). The CellsDirect™ One-Step qRT-PCR Kit may also be used to detect genomic DNA targets.

CellsDirect™ One-Step qRT-PCR Kits may be used in conjunction with hydrolysis probes (e.g. TaqMan®) or LUX™ fluorogenic primers.

Note: This kit includes 2X Reaction Mix containing ROX™ Reference Dye. An equivalent kit (11753-500), containing a separate tube of ROX™ Reference Dye, is also available.

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