规格
Accession Number:	NM_004720
Assay Entry:	Cell-based beta-arrestin recruitment
Cell Line:	U2OS
Cell State:	Dividing Cells
Cytoplasmic Tail:	Tail from AVPR2 GPCR
Druggable Target:	GPCRs
Gene Symbol:	EDG4
Population Status:	Pool of Stable Cells
Product Line:	Tango™
Reporter Gene:	BLA (Beta-Lactamase)
System Type:	Tango™
Target Entry:	EDG4
Detection Method:	Fluorescent

储存
(LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol). Includes: o Tango™ EDG4-bla U2OS cells o Tango™ EDG4-bla U2OS Assay Protocol o Certificate of Analysis The Tango™ EDG4-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.

描述

The Tango™ EDG4-bla U2OS cells contain the human Endothelial Differentiation Gene 4 (EDG4) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.

The Tango™ EDG4-bla U2OS DA cells have been functionally validated for Z' factor and EC50 concentrations of LPA (18:1). In addition, Tango™ EDG4-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section.

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