规格
Final Product Type:	mRNA
For Use With (Application):	Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), cDNA Library Construction
For Use With (Equipment):	Robotic Liquid Handling Systems
Purification Target:	mRNA
Sample Type:	Mammalian Cells, Tissue (Mammalian), Total RNA, Whole Blood
Format:	96-well plate
Isolation Technology:	Oligo dT Affinity
No. of Reactions:	960 Preps
Quantity:	10
Elution Volume Max:	80 µl
Elution Volume Min:	80 µl
High-throughput Compatibility:	High-throughput Compatible, Automated Protocols
Purification Time:	1.5 hrs
Shipping Condition:	Room Temperature
System Type:	mRNA Catcher
Product Line:	mRNA CATCHER™

储存
• 10 96-well mRNA Catcher™ PLUS plates (4°C) • 40 ml 2X Lysis Buffer L20 (room temperature) • 2 × 150 ml Wash Buffer W15 (room temperature) • 40 ml Dilution Buffer DL1 (room temperature) • 80 ml Elution Buffer E3 (room temperature) • 1 ml 0.5M DTT (4°C)

描述

The mRNA Catcher™ PLUS Kit enables high-throughput purification of mRNA from cells, tissues, blood, and total RNA in a 96-well format. By taking advantage of Locked Nucleic Acid (LNA) technology, the mRNA Catcher™ PLUS Kit provides improved performance and a faster protocol, while preserving mRNA yield and purity (see table). The mRNA Catcher™ PLUS Kit offers:

• Robust performance—high binding capacity per well (>80 ng/well)
• Reproducible results—low CVs from well-to-well and plate-to-plate (~2%)
• Optimized protocols—for small sample sizes (100 cells, 4 mg tissue, and 40 µL blood)
• Compatible plate design—may be used with most PCR cyclers

How it works
mRNA from total RNA, cells, tissue, and blood samples is hybridized to an immobilized, single-stranded 20-mer oligo(dT)-containing LNA. The incorporation of LNA into the oligo probe increases the hybridization efficiency and specificity of the mRNA binding. The mRNA can either be eluted or directly reversed-transcribed to cDNA directly in the well. The mRNA Catcher™ PLUS method does not require centrifugation, precipitation, phase separation, or column filtration. In addition, the entire method is amenable to automation for streamlined, high-throughput expression profiling for large-scale processes. Isolated mRNA is suitable for use in downstream applications such as RT-PCR and qRT-PCR.

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