规格
Assay:	Kinase Binding Assay
Assay Entry:	Biochemical binding/kinetic
Detection Method:	Fluorescent
Excitation/Emission:	340⁄665
For Use With (Equipment):	Microplate Reader
Product Line:	LanthaScreen®
Readout:	Kinetic
Shipping Condition:	Dry Ice
Target Entry:	STK33, NEK2, MAP3K7/MAP3K7IP1, CSNK1G1, IRAK4, HIPK3, CHEK2, CSNK1G2, CSNK1E, DAPK2
Target Enzyme:	Kinases (Multiple)
Technique:	TR-FRET
Label or Dye:	Alexa Fluor™ 647
Color:	Red
Format:	384-well plate

储存
Store at -20°C

描述

Kinase Tracer 222 is an Alexa Fluor® 647 dye-labeled, ATP-competitive kinase inhibitor scaffold for use in the LanthaScreen® Eu Kinase Binding Assay.

The LanthaScreen® Eu Kinase Binding Assay
• Is easily optimized
• Measures inhibitor binding
• Performs simple off-rate measurements

Easily Screen for Kinase Binding
The LanthaScreen® Eu Kinase Binding Assay is a TR-FRET assay that lets you measure inhibitor binding to non-activated kinases, perform simple off-rate measurements, and makes simple the study of type III (allosteric) inhibitors.

The LanthaScreen® Eu Kinase Binding Assay is easy to optimize and run. The assay uses a simple mix-and-read format with a single one hour incubation and no development steps. Binding of an Alexa Fluor® conjugate or “tracer” to a tagged kinase is detected by addition of a europium-labeled anti-tag antibody, in this case anti-GST. Simultaneous binding of the tracer and antibody to the kinase results in a high degree of fluorescence resonance energy transfer (TR-FRET), while displacement of the tracer with a kinase inhibitor results in a loss of TR-FRET.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Related Link
• Learn more about the LanthaScreen® Eu Binding Assay.

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