规格
Cloning Method:	Restriction Enzyme ⁄ MCS
Key Functions:	Constitutive Expression
Constitutive or Inducible System:	Constitutive
Delivery Type:	Transfection
Promoter:	SV40
Selection Agent (Eukaryotic):	Zeocin™
Vector:	pZeo
Protein Tag:	Untagged

储存
20 µg pZeoSV2 (+) or (-) and 20 µg pZeoSV2/lacZ control vector (20 µg) are supplied supercoiled and lyophilized, 3 x 1.25 ml Zeocin™ is supplied at 100 mg/ml. Store all components at -20°C. Zeocin™ should be stored away from light. Vectors and Zeocin™ are guaranteed stable for 6 months when properly stored.

描述

pZeoSV2(+) and (-) are 3.5-kb vectors designed for high-level, constitutive expression in mammalian cell lines that express the SV40 large T antigen. The Zeocin™ resistance gene provides fast and efficient selection in E. coliand mammalian cells that do not express the SV40 large T antigen. The vectors have the following features which make them efficient, easy-to-use tools:

• SV40 enhancer-promoter and origin for high-level, constitutive expression and replication in mammalian cells
• BGH polyA signal for efficient processing of mRNA transcripts
• Multiple cloning site in the forward (+) and reverse (-) orientations for easier cloning
• Small size for efficient cloning
• f1 origin for the rescue of single-strand DNA (sense strand)
• pUC origin of replication for growth in E. coli

Expression of the Zeocin™ resistance gene is driven by the CMV promoter in mammalian cell lines and by the synthetic EM-7 promoter in E. coli. pZeoSV2 can be used in transient expression assays as well as to create stable cell lines.

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