规格
|
Assay:
|
Serine⁄Threonine Kinase Assay |
Assay Entry:
|
Biochemical phospho-specific activity (no antibody) |
Excitation/Emission:
|
405⁄465, 535 |
No. of Reactions:
|
800 Reactions |
Product Line:
|
Z´-LYTE® |
Readout:
|
End Point |
References:
|
JBC(2001) 276, p38956 |
Shipping Condition:
|
Dry Ice |
Source:
|
myosin light chain 2 (KKRPQRRYSNVF) |
Substrate Properties:
|
Peptide-Based Substrate |
Target Entry:
|
ROCK2, MYLK2, DAPK3, CDC42BPA, CDC42BPB, CAMK4 |
Target Enzyme:
|
Serine⁄Threonine Kinases |
Technique:
|
FRET |
Validated Kinases:
|
CAMK4, CDC42BPA, CDC42BPB, DAPK3, MYLK2, ROCK2 |
Label or Dye:
|
Methylcoumarin |
Format:
|
384-well plate |
储存
|
Z´-LYTE® Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions. |
A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon myosin light chain 2, containing Ser-20 and a Hyd-X-R-X-X-(S⁄T) consensus sequence, where Hyd is a hydrophobic residue and X is any residue.