- 商品介绍
- 规格参数
- 包装参数
|
规格 |
|
| Bacterial or Yeast Strain: | TOP10 |
| Blue/White Screening: | Yes |
| Cloning Methylated DNA: | Yes |
| Cloning Unstable DNA: | Not Suitable for cloning unstable DNA |
| Competent Cell Type: | Electrocompetent |
| F'episome (to make ssDNA): | Lacks F' Episome |
| Format: | One Shot |
| High-throughput Compatibility: | Not High-throughput Compatible (Manual) |
| Improves Plasmid Quality: | Yes |
| Plasmid: | May be used for plasmids > 20 kb |
| Preparing Unmethylated DNA: | Not Suitable for preparing unmethylated DNA |
| Product Line: | One Shot™ |
| Propagating ccdB Vectors: | Not for ccdB vector propagation |
| Quantity: | 20 reactions |
| Reduces Recombination: | Yes |
| Shipping Condition: | Dry Ice |
| Species: | E. coli |
| T1 Phage - Resistant (tonA): | No |
| Transformation Efficiency: | > 1x10^10 |
| Transformation Efficiency Level: | High Efficiency (> 10^9 cfu⁄µg) |
|
储存 |
|
|
Contains: • One Shot® TOP10 Electrocomp™ E. coli: 21 vials, 50 µl each • pUC19 DNA (10 pg/µl): 1 vial, 50 µl • S.O.C. medium: 1 bottle, 6 ml Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature. |
|
描述 |
One Shot® TOP10 Electrocomp™ E. coli are provided at a transformation efficiency of 1 x 1010 cfu/ µg supercoiled DNA and are ideal for high-efficiency cloning and plasmid propagation. They allow stable replication of high copy number plasmids. The genotype of TOP10 cells is similar to that of the DH10B™ strain, and offers the following features:
• hsdR for efficient transformation of unmethylated DNA from PCR amplifications
• mcrA for efficient transformation of methylated DNA from genomic preparations
• lacZΔM15 for blue/white color screening of recombinant clones
• endA1 for cleaner preparations of DNA—get better results in downstream applications due to the elimination of nonspecific digestion by Endonuclease I
• recA1 for reduced occurrence of nonspecific recombination in cloned DNA
Kit options
One Shot® TOP10 Kits are available with either chemically competent or electrocompetent E. coli to fit your specific transformation needs. TOP10 E. coli are also available in the high-throughput MultiShot™ format.
| 长度(mm) | |
| 宽度(mm) | |
| 高度(mm) | |
| 重量(kg) |
-
询价
-
询价
-
询价
-
询价
-
询价
-
询价
-
询价
-
¥ 3498.00
-
询价
-
询价
-
询价
-
询价
-
询价
-
询价
-
询价
-
询价
-
询价
-
¥ 70526.15
-
¥ 75435.65
-
¥ 21144.84
微信小程序
7X24小时在线咨询