PepTivator^® RAS G12D is a pool of lyophilized peptides, consisting of 15-mer, 10-mer, and 9-mer sequences containing the G12D mutation. G12D is a common driver mutation that occurs with high frequency across various human cancer types.

PepTivator RAS G12D covers the mutated protein domain [MTEYKLVVVGADGVGKSALTIQLIQN] and contains every 15-mer, 10-mer, and 9-mer peptide bearing the mutated amino acid. This diversity allows loading of HLA without prior knowledge of alleles. The proteins H-Ras, N-Ras, and K-Ras share 90% identity in their G domain (residues 1–166), and this peptide pool covers the respective mutation for all three isoforms.

The PepTivator RAS G12D hse been developed for the efficient in vitro stimulation of RAS G12D–specific CD4⁺ and CD8⁺ T cells. Peptides of 15-, 10-, and 9 amino acids in length, represent an optimal solution for stimulating both CD4⁺ and CD8⁺ T cells in various applications, as well as providing the best coverage of potential HLA alleles.

Quantitative, phenotypical, or functional analysis of RAS G12D–specific T cell immunity can provide important information on the natural course of immune responses.

Background information

RAS or KRAS (Kirsten rat sarcoma virus; UniProtKB Acc. no P01116), belongs to a family of proteins that are ubiquitously expressed in all animal cell types and is composed of 189 amino acids (aa). It is involved in cell growth, differentiation, and survival and the mutations in RAS genes, here G12D, can lead to different cancer formation acting as oncogenes. PepTivator RAS G12D covers the above mentioned mutation in all three isoforms of Ras: K-Ras, N-Ras, and H-Ras. The RAS mutations are found within approximately 20–30% of all human cancers. Cancers with very high RAS mutational burden include pancreatic cancer (90%), colorectal cancer (55%), and non-small cell lung cancer (30%).

长度(mm)
宽度(mm)
高度(mm)
重量(kg)