规格
Cloning Method:	Restriction Enzyme ⁄ MCS
Key Functions:	Constitutive Expression
Constitutive or Inducible System:	Constitutive
Delivery Type:	Transfection
Promoter:	CMV
Selection Agent (Eukaryotic):	Blasticidin
Vector:	pCMV
Protein Tag:	Untagged

储存
20 µg of a BsdCassette™ vector is provided supercoiled and lyophilized. The BsdCassette™ Starter Kits include 20 µg of BsdCassette™ vector and 50 mg of Blasticidin. Store the vector and Blasticidin at -20°C. All reagents are guaranteed stable for 6 months when properly stored.

描述

Blasticidin is a potent selection agent that allows selection of stable mammalian cell lines in as little as one week. The BsdCassette™ vectors simplify the construction of custom vectors that use Blasticidin resistance as a selectable marker. Each BsdCassette™ vector is designed for use in a specific host system and offers:

• A strong host promoter to drive expression of the Blasticidin resistance gene (bsd)(Table 1)
• Two large polylinkers flanking the promoter and bsd gene to facilitate the construction of Blasticidin resistant vectors

Each BsdCassette™ vector carries the synthetic EM-7 promoter for expression of the bsd gene in E. coli. This eliminates the need to add a second antibiotic resistance gene for selection in E. coli. BsdCassette™ vectors provide a eukaryotic promoter for expressionin yeast (pTEF1/Bsd) or mammalian cells (pCMV/Bsd, pEF1/Bsd, pUB/Bsd) for construction of expression vectors with Blasticidin selection for a wide range of host cell types.

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